9UCT

beta-glucosidase GH1-family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	113
Detector technology	PIXEL
Collection date	2022-03-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.1
Spacegroup name	P 21 21 21
Unit cell lengths	74.603, 96.871, 143.005
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.110 - 1.900
R-factor	0.1556
Rwork	0.155
R-free	0.18030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z1h
RMSD bond length	0.008
RMSD bond angle	0.967
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	142431	1466
<I/σ(I)>	22.05
Completeness [%]	95.6
Redundancy	11.4
CC(1/2)	0.996	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG4000, 2-propanol