9U9D

Bipartite Genetically Encoded Biosensor sG-GECO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	51.104, 51.104, 201.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.140 - 1.800
R-factor	0.1868
Rwork	0.183
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.140	1.870
High resolution limit [Å]	1.800	1.800
Number of reflections	634405	25780
<I/σ(I)>	19.93
Completeness [%]	99.9
Redundancy	24.6
CC(1/2)	0.999	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2m sodium malonate dibasic monohydrate, 0.1M Bis-Tris propane pH 8.5, 20% w/v PEG 3350