9U98
Glycoside hydrolase family 1 beta-glucosidase (E318G mutant) from Streptomyces griseus (sophorose complex)
Replaces: 8KAQExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.793, 100.179, 96.048 |
| Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
| Resolution | 46.818 - 2.130 |
| Rwork | 0.181 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.666 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.824 | 2.170 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 93387 | 4558 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.995 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris propane (pH 6.5), 0.3 M sodium formate, 18% PEG 3350 |
