9U4S

Crystal Structure of PI5P4K-gamma in complex with n40

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-09-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 2 21
Unit cell lengths	263.910, 47.760, 66.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.230 - 2.250
R-factor	0.28
Rwork	0.279
R-free	0.30360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.781
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.230	2.310
High resolution limit [Å]	2.250	2.250
Rmerge	0.312	2.906
Rmeas	0.325	3.025
Rpim	0.091	0.833
Total number of observations	531990	38355
Number of reflections	41016	2965
<I/σ(I)>	7.1	1.1
Completeness [%]	100.0
Redundancy	13	12.9
CC(1/2)	0.992	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium citrate tribasic monohydrate, 20% w/v Polyethylene glycol 3350