9TWS
Complex of E. coli leucyl-tRNA synthetase AN3365-resistant mutant T247I bound to tRNA(leu)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-15 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.766, 119.354, 140.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.900 - 2.463 |
| R-factor | 0.2125 |
| Rwork | 0.210 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3n |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.820 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.900 | 2.640 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rpim | 0.034 | 0.504 |
| Number of reflections | 39113 | 1956 |
| <I/σ(I)> | 12.1 | 1.4 |
| Completeness [%] | 94.5 | 65 |
| Redundancy | 5.9 | 6.7 |
| CC(1/2) | 0.995 | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M KH2PO4, 19-23% PEG 3350 |
