9TWR
Complex of E. coli leucyl-tRNA synthetase AN3365-resistant mutant R344H bound to tRNA(leu) and AN3365
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-15 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.504, 119.177, 142.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.420 - 2.060 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.830 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.430 | 2.280 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rpim | 0.050 | 0.475 |
| Number of reflections | 56776 | 2839 |
| <I/σ(I)> | 8.7 | 1.6 |
| Completeness [%] | 95.1 | 68.2 |
| Redundancy | 5.4 | 4.6 |
| CC(1/2) | 0.994 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M KH2PO4, 19-23% PEG 3350 |
