9TP8

Crystal structure of the C-terminal Ser/Thr phosphatase D510N H707K mutant of the Kelch phosphatase BSU1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000031
Spacegroup name	C 1 2 1
Unit cell lengths	139.757, 44.047, 79.241
Unit cell angles	90.00, 121.07, 90.00

Refinement procedure

Resolution	41.340 - 1.600
R-factor	0.1617
Rwork	0.161
R-free	0.18240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.440
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.340	1.690
High resolution limit [Å]	1.600	1.600
Rmeas	0.079	1.373
Number of reflections	106610	17189
<I/σ(I)>	11.66	1.09
Completeness [%]	99.0	98.6
Redundancy	3.4	3.3
CC(1/2)	0.999	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	1.6 M sodium / potassium phosphate (pH 7.0)