9TOY
Investigating the binding mechanism of Interferon Regulatory Factor 4 to DNA in the context of Multiple Myeloma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-07-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.512, 65.116, 70.694 |
| Unit cell angles | 89.86, 75.32, 66.79 |
Refinement procedure
| Resolution | 59.556 - 2.100 |
| Rwork | 0.211 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.877 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.556 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 70231 | 2415 |
| <I/σ(I)> | 11.14 | |
| Completeness [%] | 95.5 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.976 | 0.363 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2M Calcium Acetate, 0.1M MES pH 6, 20% PEG 8000 |
