9TM6
Structure of duck RIG-I (delta CARDs) bound to 31-mer RNA mismatched hairpin with 5'pppG-C blunt end mimicking the influenza A virus vRNA promoter (panhandle) and to ADP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.865, 99.594, 192.218 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.109 - 1.982 |
| Rwork | 0.200 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.166 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.109 | 2.236 |
| High resolution limit [Å] | 1.982 | 1.982 |
| Rmerge | 0.165 | 1.359 |
| Number of reflections | 35585 | 1779 |
| <I/σ(I)> | 9.8 | 1.6 |
| Completeness [%] | 60.5 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.978 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.2 M Ammonium formate 20 % PEG 3350 |
