9TLX
Crystal structure of Brugia malayi DAF-12 ligand binding domain in complex with a coactivator peptide and delta4-dafachronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-13 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.293, 77.750, 90.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.100 - 1.700 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.258 | |
| Rmeas | 0.365 | |
| Number of reflections | 60374 | 11830 |
| <I/σ(I)> | 10.71 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium citrate dibasic, 20% (w/v) PEG 3350 |
