9TL4
Crystal structure of Haemonchus contortus DAF-12 ligand binding domain in complex with a coactivator peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-13 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.519, 74.233, 92.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.520 - 2.000 |
| R-factor | 0.1799 |
| Rwork | 0.178 |
| R-free | 0.21280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.460 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.520 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.033 | |
| Rmeas | 0.046 | |
| Number of reflections | 33162 | 1816 |
| <I/σ(I)> | 14.49 | |
| Completeness [%] | 90.9 | 59.6 |
| Redundancy | 1.9 | |
| CC(1/2) | 0.999 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.4 M sodium malonate pH7 |
