9TL1
Structure of duck RIG-I (delta CARDs) bound to 31-mer RNA mismatched hairpin with 5'ppA-U blunt end mimicking the influenza B virus vRNA promoter (panhandle) and to ADP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.883, 100.026, 190.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.064 - 1.704 |
| Rwork | 0.185 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.593 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.064 | 1.859 |
| High resolution limit [Å] | 1.704 | 1.704 |
| Rmerge | 0.109 | 1.024 |
| Number of reflections | 70381 | 3519 |
| <I/σ(I)> | 8.6 | 1.5 |
| Completeness [%] | 77.0 | |
| Redundancy | 16.9 | |
| CC(1/2) | 0.922 | 0.566 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | Na-citrate pH 5.5 PEG 3000 22 % NDSB 201 0,1M |
