9TJX

Structure of factor VII Gla domain bound to EPCR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XAIRA
Synchrotron site	ALBA
Beamline	XAIRA
Temperature [K]	295
Detector technology	PIXEL
Collection date	2025-11-21
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.97923
Spacegroup name	C 1 2 1
Unit cell lengths	278.406, 44.222, 111.252
Unit cell angles	90.00, 93.01, 90.00

Refinement procedure

Resolution	43.670 - 3.000
R-factor	0.2442
Rwork	0.242
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.613
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.670	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.153	0.480
Rmeas	0.187	0.583
Rpim	0.106	0.326
Number of reflections	25876	4440
<I/σ(I)>	4.2	2.2
Completeness [%]	93.0
Redundancy	3
CC(1/2)	0.967	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Ammonium sulfate, 0.1 M Tris PH 8.5, 12 % w/v PEG 8000