9TFU
Crystal structure of beta-TrCP bound by monophosphorylated ATF4 degron peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.986, 47.146, 150.913 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.460 - 1.997 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.456 | 2.170 |
| High resolution limit [Å] | 1.996 | 1.996 |
| Number of reflections | 16038 | 802 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 90.2 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.784 | 0.661 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEGMME2K, 0.1 M Bis-tris pH 6.5 |
