9T8Y
Crystal structure of beta-TrCP bound by monophosphorylated NRF2 degron peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.916, 47.090, 151.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.660 - 1.795 |
| R-factor | 0.2005 |
| Rwork | 0.197 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.060 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.662 | 1.992 |
| High resolution limit [Å] | 1.795 | 1.795 |
| Number of reflections | 19413 | 971 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 90.0 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.881 | 0.213 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 19% PEG3350, 0.1 M PCTP pH 6.2 |
