9T4N
Structure of the Q318A mutant of the SUN4 domain from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.400, 99.230, 102.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.220 - 1.280 |
| R-factor | 0.1428 |
| Rwork | 0.141 |
| R-free | 0.16840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.370 | 1.350 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.049 | 0.555 |
| Number of reflections | 81024 | 8041 |
| <I/σ(I)> | 11.8 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 281 | 50 mg/ml, 0.1 MES, 40% PEG400, 0.2 M MgCl2 |
