9T38

Rhs2-CT endonuclease toxin in complex with cognate immunity protein RhsI2 and EF-Tu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-21
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	61.598, 110.569, 113.863
Unit cell angles	90.00, 102.39, 90.00

Refinement procedure

Resolution	60.236 - 2.450
Rwork	0.270
R-free	0.31380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.265
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.236	2.520
High resolution limit [Å]	2.450	2.450
Rmerge	0.151	1.721
Rpim		1.134
Number of reflections	54856	4485
<I/σ(I)>	6
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.988	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		296	0.2 uL of 18 mg/mL Rhs2-ct-RhsI2-EF-Tu (in 20 mM Tris-HCl pH 7.5, 250 mM NaCl, 2 mM MgCl2, 2 mM GDP and 0.5 mM TCEP) mixed with 0.2 uL of reservoir solution (0.2 M ammonium actetate, 0.1 M HEPES pH 7.5 and 25% PEG3350). Cryoprotected with Paratone N