9T2I
SP100 nuclear antigen with crystallization epitope mutations N816D:T820R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.090, 45.730, 83.950 |
| Unit cell angles | 90.00, 102.37, 90.00 |
Refinement procedure
| Resolution | 56.191 - 1.350 |
| Rwork | 0.158 |
| R-free | 0.18520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.783 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431 (refmacat 0.4.126)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.360 |
| Rmerge | 0.041 | 1.006 |
| Number of reflections | 100552 | 7176 |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 96.0 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 0.2M ammonium sulfate 25% PEG3350 0.1M bis-tris pH 6.5 |
