9SZQ
Crystal structure of the E336K mutant of human Apoptosis Inducing Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.283, 89.099, 60.110 |
| Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
| Resolution | 50.160 - 1.800 |
| R-factor | 0.16971 |
| Rwork | 0.168 |
| R-free | 0.20285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.796 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.100 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.534 |
| Number of reflections | 47011 | 6861 |
| <I/σ(I)> | 8.4 | 2.6 |
| Completeness [%] | 96.2 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | PEG 6000, TrisHCl pH 8.5, lithium sulfate |
