9SYN

Crystal structure of TEAD4 in complex with Vgll1-peptide and IAG933

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999802
Spacegroup name	P 21 21 21
Unit cell lengths	45.359, 68.267, 70.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.700 - 1.300
R-factor	0.2077
Rwork	0.206
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.090	1.340
High resolution limit [Å]	1.290	1.290
Rmerge	0.059	0.704
Rmeas	0.062	0.751
Rpim	0.020	0.255
Number of reflections	43428	2171
<I/σ(I)>	15.3	2.5
Completeness [%]	78.2	40.3
Redundancy	9.8	8.3
CC(1/2)	0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium sulfate, HEPES, PEG3350