9SR0

Mouse vinculin head domain 1 (VD1) (residues 2-258) with A50I mutation in complex with mouse talin 1 helix 50 (H50, residues 2072-2103)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-26
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.6199
Spacegroup name	P 21 21 21
Unit cell lengths	43.700, 75.800, 82.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.920 - 1.490
R-factor	0.1917
Rwork	0.190
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.680
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.630	1.520
High resolution limit [Å]	1.490	1.490
Rmerge	0.078
Number of reflections	45494	2262
<I/σ(I)>	10.1
Completeness [%]	99.7
Redundancy	7.4
CC(1/2)	0.999	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	292.15	100 mM Bis-tris pH 6.0, 15% PEG 3350