9SPQ

p53 cancer mutant V272M in complex with DARPin C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	38.041, 93.877, 53.552
Unit cell angles	90.00, 109.75, 90.00

Refinement procedure

Resolution	46.940 - 2.200
R-factor	0.17743058057
Rwork	0.175
R-free	0.22243
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.825
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.135	0.901
Number of reflections	17985	1558
<I/σ(I)>	8.6	2
Completeness [%]	99.7	99.8
Redundancy	5.1	5.2
CC(1/2)	0.995	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein solution: 0.9 mg/ml p53-DARPin complex in 25 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP. Reservoir buffer: 2.4 M sodium malonate. Drop volume ratio 2:1.