9SPP
p53 cancer mutant Y234C in complex with DARPin C10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.737, 93.773, 53.223 |
| Unit cell angles | 90.00, 110.20, 90.00 |
Refinement procedure
| Resolution | 46.900 - 1.470 |
| R-factor | 0.157360349453 |
| Rwork | 0.156 |
| R-free | 0.19207 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.051 | 0.799 |
| Rpim | 0.025 | 0.398 |
| Number of reflections | 58708 | 2899 |
| <I/σ(I)> | 15.5 | 2.1 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 5 | 5.2 |
| CC(1/2) | 0.999 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein solution: 1.5 mg/ml p53-DARPin complex in 25 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP. Reservoir buffer: 15% PEG3350, 0.1 M magnesium formate. Drop volume ratio 2:1. |
