9SGH

Bimekizumab Fab in complex with human interleukin-17A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99989
Spacegroup name	P 21 21 2
Unit cell lengths	117.562, 148.492, 92.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.090 - 2.661
R-factor	0.2228
Rwork	0.221
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	autoPROC (1.1.7 (20230726))
Data scaling software	autoPROC (1.1.7 (20230726))
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (26-JUL-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.755	2.918
High resolution limit [Å]	2.661	2.661
Rmerge	0.277	2.291
Rmeas	0.291	2.380
Rpim	0.086	0.640
Number of reflections	33970	1698
<I/σ(I)>	5.8	1.4
Completeness [%]	93.9	58.8
Redundancy	10.6	13.1
CC(1/2)	0.998	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.2M zinc acetate, 0.1M imidazole pH 6.5, 10% PEG 8,000