9SAH

Structure of a fusion of biosynthetic proteins E4 and NB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 21
Unit cell lengths	50.840, 117.810, 154.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.280 - 2.200
R-factor	0.2338
Rwork	0.232
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4 (10-JUL-2024))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.280	2.330
High resolution limit [Å]	2.200	2.200
Rmeas	0.320	2.000
Number of reflections	47991	7640
<I/σ(I)>	9.59
Completeness [%]	100.0	100
Redundancy	13.52	13.16
CC(1/2)	0.990	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	magnesium nitrate and PEG3350