9S9R
Crystal structure of p53 cancer mutant Y220N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.055, 71.019, 104.826 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.970 - 1.700 |
| R-factor | 0.187481128669 |
| Rwork | 0.185 |
| R-free | 0.22865 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.754 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.970 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.084 | 0.938 |
| Number of reflections | 53010 | 2814 |
| <I/σ(I)> | 10.2 | 2.1 |
| Completeness [%] | 98.4 | 99.4 |
| Redundancy | 5.5 | 5.6 |
| CC(1/2) | 0.998 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 22.5% (w/v) BMW PEG smear broad, 0.1 M potassium sodium tartrate, 10% (w/v) ethylene glycol, 0.1 M sodium cacodylate, pH 5.3-5.8 |
