9S8W

Human heparanase in complex with neutralizing antibody A54 Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91589
Spacegroup name	P 41 21 2
Unit cell lengths	69.738, 69.738, 429.781
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.440 - 3.490
Rwork	0.220
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.813
Data reduction software	xia2
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.440	3.870
High resolution limit [Å]	3.490	3.490
Rmeas	0.280	2.250
Number of reflections	9227	452
<I/σ(I)>	9.2	1.7
Completeness [%]	86.8
Redundancy	15
CC(1/2)	0.999	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium Nitrate 20% PEG3350