9S8A
Amuc0451_S1_20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-31 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 113.660, 59.900, 123.720 |
| Unit cell angles | 90.00, 115.75, 90.00 |
Refinement procedure
| Resolution | 51.754 - 1.600 |
| Rwork | 0.156 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.681 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.900 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 1291159 | 55288 |
| <I/σ(I)> | 10.3 | 1.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.998 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20 % w/v PEG 3350, 0.1 M Bis-Tris propane 7.5, 0.02 M Sodium/potassium phosphate 40 mg/ml Amuc0451 |
