Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-29 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0596 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.900, 73.710, 55.860 |
| Unit cell angles | 90.00, 94.78, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.15765 |
| Rwork | 0.156 |
| R-free | 0.18722 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.079 | 0.788 |
| Number of reflections | 49669 | 8828 |
| <I/σ(I)> | 9.47 | 1.99 |
| Completeness [%] | 96.7 | 95 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.1 M sodium acetate 17 % (w/v) PEG 3350 |
