9S2X
Crystal structure of the human RAGE ectodomain in complex with murine S100A6 mutant Y84C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.965 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 75.650, 113.010, 139.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.907 - 2.350 |
| R-factor | 0.2059 |
| Rwork | 0.204 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.639 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmeas | 0.081 | 0.884 |
| Number of reflections | 25103 | 13848 |
| <I/σ(I)> | 15.37 | 2.1 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2 M Zn acetate, 0.1 M Na cacodylate pH 6.5, 9% isopropanol |
