9S0V

The Crystal Structure of Human Tissue Nonspecific Alkaline Phosphatase (hTNAP) in complex with phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-16
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967700
Spacegroup name	C 2 2 21
Unit cell lengths	156.730, 297.700, 205.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.560 - 3.350
R-factor	0.2178
Rwork	0.215
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.944
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.560	9.880	3.550
High resolution limit [Å]	3.350	7.050	3.350
Rmeas	0.400	0.079
Number of reflections	69299	2869	10753
<I/σ(I)>	7.25		2.17
Completeness [%]	99.2	98.6	96.6
Redundancy	14	13.7	12.2
CC(1/2)	0.992	0.999	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium phosphate, 20% (w/v) PEG 3350