9RRY

Crystal structure of the human METTL3-METTL14 in complex with compound 1 (AD-79)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	63.980, 63.980, 226.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.650 - 1.910
R-factor	0.2226
Rwork	0.221
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.230	2.030
High resolution limit [Å]	1.910	1.910
Number of reflections	42626	6766
<I/σ(I)>	14.45
Completeness [%]	99.9
Redundancy	8.18
CC(1/2)	0.999	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 0.4M Magnesium acetate