9RIB
Crystal structure of C278S mutant of mouse CDC14A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.73 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.507, 158.086, 69.156 |
| Unit cell angles | 90.00, 124.56, 90.00 |
Refinement procedure
| Resolution | 28.590 - 1.620 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.076 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.043 | 1.835 |
| High resolution limit [Å] | 1.619 | 1.619 |
| Rmerge | 0.118 | 1.008 |
| Rpim | 0.047 | 0.400 |
| Number of reflections | 61433 | 3072 |
| <I/σ(I)> | 9.2 | 1.7 |
| Completeness [%] | 53.5 | 8.6 |
| Redundancy | 7.2 | |
| CC(1/2) | 0.996 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium sulfate; Polymer: 20w/v polyethylene glycol 3350; Buffer: 0.1M Bis-Tris propane, pH 6.5 |
