9RD5
Phi3T SroF bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-24 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.182, 115.618, 154.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.350 - 2.500 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.708 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 154.370 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.244 | |
| Rmeas | 0.264 | |
| Number of reflections | 23699 | 2640 |
| <I/σ(I)> | 25.3 | |
| Completeness [%] | 100.0 | 99.3 |
| Redundancy | 13 | 13.3 |
| CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294.15 | 25 % PEG 4000, 0.1 M Tris pH 8.5 and 200 mM CaCl2 |
