9RC2
DvhD, periplasmic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-25 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 120.380, 120.380, 123.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.050 - 3.000 |
| R-factor | 0.2733 |
| Rwork | 0.270 |
| R-free | 0.30180 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.765 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 20145 | 1437 |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 21.1 | |
| CC(1/2) | 1.000 | 0.668 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium acetate, 0.1 M Bis-Tris pH 6, 27% polyethylene glycol 3,350, 20% xylitol |
