9R8Z

METTL3/14 complex with switchable fragment

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91587
Spacegroup name	P 41 21 2
Unit cell lengths	100.830, 100.830, 117.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.570 - 2.210
Rwork	0.174
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	2.594
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.88))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.570	2.270
High resolution limit [Å]	2.210	2.210
Number of reflections	31097	2250
<I/σ(I)>	14.7
Completeness [%]	100.0	100
Redundancy	13	13.6
CC(1/2)	0.999	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	10% w/v PEG 4000, 20% v/v glycerol, 0.1 M bicine/Trizma base pH, 8.5 0.02 M 1,6-hexanediol, 0.02 M 1-butanol, 0.02 M (RS)-1,2- propanediol, 0.02 M 2-propanol, 0.02 M 1,4-butanediol, 0.02 M 1,3-propanediol