9R0E
Structure of the human heterotetrameric cis-prenyltransferase complex harboring NgBR-S249T in complex with magnesium, FsPP and IPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 184.658, 184.658, 112.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.160 - 2.820 |
| R-factor | 0.2425 |
| Rwork | 0.240 |
| R-free | 0.28990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.684 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.164 | 2.990 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmeas | 0.192 | 2.983 |
| Number of reflections | 17888 | 2849 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1 M Hepes pH 8, 30% Pentaerythritol ethoxylate, 4% PVP K15, 30% (w/v) 1,6-Hexanediol |
