9QT2
Bacterial histone HLp from Leptospira perolatii bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 48.769, 91.613, 100.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.560 - 1.900 |
| R-factor | 0.24269 |
| Rwork | 0.235 |
| R-free | 0.28794 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.560 | 2.050 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.060 | 1.534 |
| Number of reflections | 6179 | 364 |
| <I/σ(I)> | 13.86 | 1.34 |
| Completeness [%] | 68.4 | 20 |
| Redundancy | 7.51 | 6.44 |
| CC(1/2) | 1.000 | 0.426 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Carboxylic acids, 0.1 M Morpheus Buffer System 3, pH 8.5 and 50% (v/v) Morpheus Precipitant Mix 4 |
