9QRV
Crystal Structure of an anti-VWF CK Domain Fab in Complex with the C-Terminal CK Domain of Cynomolgus Monkey von Willebrand Factor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.976, 116.327, 252.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.120 - 2.500 |
| R-factor | 0.2182 |
| Rwork | 0.217 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.970 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.230 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | |
| Rpim | 0.030 | |
| Number of reflections | 33666 | 3728 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.5 | |
| CC(1/2) | 0.990 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M Bis-Tris pH 5.5, 2 M ammonium sulfate |
