Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.965 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 70.492, 70.492, 205.594 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.940 - 1.800 |
| R-factor | 0.19074 |
| Rwork | 0.188 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.783 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.820 | 0.440 |
| Number of reflections | 53382 | 2693 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.03 M citric acid, 0.045 M BIS-TRIS propane (pH 6.4), 15% (wt/vol) polyethylene glycol 3350, and 25% (vol/vol) glycerol |
