9QKQ
Crystal structure of hTEAD4 YAP binding domain (hTEAD4-YBD) in complex with peptide 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-28 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.88561 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.538, 40.243, 97.892 |
| Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
| Resolution | 97.780 - 1.740 |
| R-factor | 0.18499 |
| Rwork | 0.183 |
| R-free | 0.22103 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.476 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.780 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.022 | 0.621 |
| Rmeas | 0.024 | 0.674 |
| Rpim | 0.010 | 0.262 |
| Number of reflections | 68607 | 3704 |
| <I/σ(I)> | 32.8 | 2.6 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 6.8 | 6.6 |
| CC(1/2) | 0.999 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.9-1.2 M sodium/potassium phosphate buffer at pH 5.6 |
