Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-21 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 62 |
| Unit cell lengths | 67.750, 67.750, 71.114 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.670 - 1.450 |
| R-factor | 0.15102 |
| Rwork | 0.150 |
| R-free | 0.16200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.356 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.670 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.079 | 1.516 |
| Rmeas | 0.081 | 1.557 |
| Rpim | 0.018 | 0.349 |
| Total number of observations | 681895 | 31898 |
| Number of reflections | 32898 | 1643 |
| <I/σ(I)> | 19.5 | 1.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.7 | 19.4 |
| CC(1/2) | 0.999 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | 2.3 M Sodium Chloride 0.1 M Sodium Citrate |
