9Q8Y

Structure of RNF38 RING with linchpin mutant R454Y in complex with Ubch5B-Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 62
Unit cell lengths	116.079, 116.079, 90.315
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.920 - 2.630
R-factor	0.2338
Rwork	0.232
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.375
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.920	2.770
High resolution limit [Å]	2.630	2.630
Rmerge	0.052	0.856
Rpim	0.020	0.308
Number of reflections	40071	879
<I/σ(I)>	22.9	2.3
Completeness [%]	99.0	85.3
Redundancy	10
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	0.2 M Sodium acetate trihydrate, 0.1 M Imidazole pH 8.0, 10% PEG 8000