9Q84

Crystal structure of Lotus japonicus CHIP13 extracellular domain in complex with chitooctaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-19
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	73.882, 66.776, 53.203
Unit cell angles	90.00, 109.35, 90.00

Refinement procedure

Resolution	48.220 - 1.410
R-factor	0.1482
Rwork	0.146
R-free	0.18140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.131
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.220	1.450
High resolution limit [Å]	1.410	1.410
Rmerge	0.064	0.998
Rmeas	0.076	1.283
Rpim	0.040	0.790
Number of reflections	86273	2307
<I/σ(I)>	7.96	0.79
Completeness [%]	94.3
Redundancy	3.5
CC(1/2)	0.999	0.316

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292.15	0.2 M NaCl, 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG-3350