9Q3Y

GluN1b-GluN2B ATD in complex with radiprodil

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.920126
Spacegroup name	C 1 2 1
Unit cell lengths	267.949, 60.672, 144.647
Unit cell angles	90.00, 116.23, 90.00

Refinement procedure

Resolution	25.000 - 2.740
R-factor	0.18827
Rwork	0.187
R-free	0.22276
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.654
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.810
High resolution limit [Å]	2.740	2.740
Rmerge	0.070	0.781
Number of reflections	52067	52067
<I/σ(I)>	10.7
Completeness [%]	98.8
Redundancy	4.1
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	4 M sodium formate, 0.1 M HEPES (pH 7.5), 35 mM NaCl, 7 mM Tris (pH 8.0)