9Q22
Crystal structure of ternary complex Helios-ZF2:I-19:CRBN:DDB1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 260.240, 260.240, 123.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 130.120 - 3.406 |
| R-factor | 0.2218 |
| Rwork | 0.220 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.120 | 3.852 |
| High resolution limit [Å] | 3.406 | 3.406 |
| Rmerge | 0.322 | 1.988 |
| Rmeas | 0.330 | 2.042 |
| Rpim | 0.073 | 0.462 |
| Number of reflections | 21894 | 1095 |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 94.9 | 67 |
| Redundancy | 20.2 | 18.6 |
| CC(1/2) | 0.997 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.148 M lithium citrate, 0.1 M Tris, pH 7.5, 19.4% PEG3350 |
