9PX5

Crystal structure of Fab 7268 in complex with MBP-TREM2 Ig domain fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000039
Spacegroup name	C 1 2 1
Unit cell lengths	119.900, 64.230, 160.800
Unit cell angles	90.00, 94.45, 90.00

Refinement procedure

Resolution	47.050 - 3.700
R-factor	0.2489
Rwork	0.248
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.491
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2-5419_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.000
High resolution limit [Å]	3.700	3.700
Number of reflections	13219	2514
<I/σ(I)>	5.3
Completeness [%]	99.2
Redundancy	7.1
CC(1/2)	0.850	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	11% PEG 8000, 240mM ammonium sulfate, 100mM MES