9PVL

Crystal Structure of Fluorophore-binding Protein JF657-bp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97935
Spacegroup name	P 1 21 1
Unit cell lengths	33.358, 123.987, 56.639
Unit cell angles	90.00, 92.28, 90.00

Refinement procedure

Resolution	33.330 - 1.440
R-factor	0.212
Rwork	0.211
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.330	1.510
High resolution limit [Å]	1.440	1.440
Rmerge	0.076	0.897
Rpim	0.035	0.430
Number of reflections	83352	12180
<I/σ(I)>	10.9	1.9
Completeness [%]	100.0	100
Redundancy	6.6	6.1
CC(1/2)	0.998	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	11 % (w/v) PEG 3350, 0.1 M HEPES pH 7.5