9PSL
Co-crystal structure of BAF60A (SMARCD1) in complex with PU.1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.750, 131.850, 75.360 |
| Unit cell angles | 90.00, 93.44, 90.00 |
Refinement procedure
| Resolution | 49.630 - 2.390 |
| R-factor | 0.22722 |
| Rwork | 0.224 |
| R-free | 0.28356 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.630 | 2.470 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.140 | 0.907 |
| Rmeas | 0.151 | 0.977 |
| Rpim | 0.057 | 0.362 |
| Total number of observations | 32683 | |
| Number of reflections | 46704 | 4576 |
| <I/σ(I)> | 10.6 | 2.2 |
| Completeness [%] | 99.7 | |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M Sodium citrate pH 6.48, 1.97M Ammonium sulfate, 2% v/v PEGMME550, 0.01M Taurine, 10 mM Ethylenediaminetetraacetic disodium salt dihydrate |
