9PPP

Structure of Alpha Appendage of AP2 bound to the extended FxDxF motif derived of CCDC32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS 3.0 MICROFOCUS
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-26
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	96.664, 96.664, 195.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.430 - 2.100
R-factor	0.17
Rwork	0.168
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.903
Data reduction software	SAINT
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.170	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.150	0.801
Rpim	0.042	0.283
Number of reflections	79167	3930
<I/σ(I)>	16.1	2.8
Completeness [%]	100.0	100
Redundancy	13.7	8.8
CC(1/2)	0.998	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM SPG pH 8.0, 25% PEG 1500